Tris也被称为THAM, Tris碱，Trizma, Tris(羟甲基)氨基甲烷。
Tris是细胞和分子生物学实验中最常用的缓冲液之一。
25℃时pKa值为8.06，有效pH范围为7.0 ~ 9.0，完全符合生理pH范围。
引言
它相对便宜，易溶于水，易于获得高度纯化的形式。
此外，Tris浓缩液可以在室温下长期保存。
Tris溶液的pH取决于温度(温度升高pH值降低0.03单位)和溶液浓度(从100 mM稀释到10 mM pH值降低0.1单位)。The pH of the Tris solution is dependent on temperature (pH value decreases by ≈0.03 unit per °C increase in temperature) and solution concentration (pH value decreases by ≈0.1 unit when diluted from 100 mM to 10 mM ).
组成
1 M Tris.Cl pH (can set any desirable pH between 6.8 to 8.5)。
You can set the desired pH (between6.8 – 9) of the solution at this point with the addition of HCl.
配置方法
- Step 1: To prepare, 1000 ml of 1 M Tris.Cl buffer, weigh out 121.14 g Tris base (molecular weight = 121.14) and transfer to a 1-liter beaker/conical flask. Dissolve Tris base in 800 ml deionized/Milli-Q water using a magnetic stirrer.
- Step 2: Adjust pH to 7.2 with concentrated HCl. The solution will have a pH between 10.0 – 11.5. Add concentrated HCl (≈ 45 ml) to adjust pH to 7.2.
- Step 3: Adjust the volume to 1000 ml with deionized/Milli-Q water.
- Step 4: 0.22 um filter.