HPLC UHPLC UPLC，这么多色谱的简称，是不是分不清楚？UPLC 或者UHPLC，是一样的，都是Ultra High Performance Liquid Chromatography。HPLC就是，High Performance Liquid Chromatography。所以本文就把UPLC和UHPLC统一成为UPLC了，主要比较UPLC和HPLC的区别。

UPLC vs HPLC

总结一下，二者的主要区别是：UPLC的柱子更细，可以实现比HPLC的更好的分离效果。

因为柱子更细以后，会带来液相泵，流速，样本消耗等对应的变化，所以下表以分子筛色谱为例，对比UPLC和HPLC的区别：

	SEC-HPLC	SEC-U(H)PLC	对比
Typical Column dimensions	7.8 x 300 mm	4.6 x 150 mm	UPLC柱子更细，更短
Particle size	3 - 10 µm	< 3 µm	UPLC柱子粒径更小
Typical Run time per injection	45 minutes	10 minutes	UPLC梯度时间更短
Solvent usage per injection	45 mL	10 mL	UPLC溶剂消耗更低
Pump pressure	4 MPa	70 MPa	UPLC的泵压更高
Sample usage	300 µg	20 µg	UPLC的样本消耗更低
(µg loading varies depending on sample and system set-up)
Peak resolution	Good	Best	UPLC的峰分辨率更好
Mobile phase change over	Normal	Fast	UPLC流动相切换更快

参考

[Ultra Performance Liquid Chromatography (UPLC)](https://www.malvernpanalytical.com/en/products/technology/liquid-chromatography/ultra-performance-liquid-chromatography#:~:text=UPLC%2C%20or%20UHPLC%20(Ultra%20High,of%20a%20compound%20or%20mixture.)